A SINGLE CELL ATLAS OF LUNG DEVELOPMENT
Led by Nick Negretti, Erin Plosa, and John Benjamin, in collaboration with Jon Kropski and Nick Banovich. 92,238 cells from E12 to P14!
AGE-DETERMINED EXPRESSION OF PRIMING PROTEASE TMPRSS2 AND LOCALIZATION OF SARS-COV-2 IN LUNG EPITHELIUM
In work led by Bryce Schuler in collaboration with Erin Plosa, John Benjamin, Meghan Kapp, Nick Banovich and Jon Kropski, we leveraged our single cell developmental atlas and human autopsy and biopsy tissue to identify a biologic rationale for why neonates and children may be less severely affected by severe COVID-19 infection.
HYPEROXIA INJURY IN THE DEVELOPING LUNG IS MEDIATED BY MESENCHYMAL EXPRESSION OF WNT5A
Using in vivo neonatal injury models, ex vivo precision cut lung slices, and in vitro 3D co-culture, we identify dysregulated expression of Wnt5A by pulmonary mesenchyme as a key driver of BPD pathogenesis.
SUCCESSFUL ESTABLISHMENT OF PRIMARY TYPE 2 ALVEOLAR EPITHELIUM WITH 3D ORGANOTYPIC CO-CULTURE
Alveolar type 2 cells are critical to lung function and lung injury repair and notoriously difficult to culture and study. This methods paper reports our new organotypic co-culture method which allows for reductionist study of epithelial-mesenchymal interactions during homeostasis and injury.
A SHARED PATTERN OF Β-CATENIN ACTIVATION IN BRONCHOPULMONARY DYSPLASIA AND IDIOPATHIC PULMONARY FIBROSIS
In a collaboration with Jon Kropksi, we identify a common B-catenin signature specific to bronchopulmonary dysplasia and idiopathic pulmonary fibrosis, underscoring the importance of understanding lung disease across the lifespan in a developmental context.
POSTTRANSLATIONAL MODIFICATION OF Β-CATENIN IS ASSOCIATED WITH PATHOGENIC FIBROBLASTIC CHANGES IN BRONCHOPULMONARY DYSPLASIA
Using a bioengineered human model of neonatal hyperoxia lung injury, we identify a role of dysregulated Wnt siganling in the fibroblastic changes observed in bronchopulmonary dysplasia.
FULL LIST OF PUBLICATIONS ON PUBMED
Want to know more about what we study? Here's the full list of publications on PubMed